{"id":18,"slug":"cryopreservation","name":"Cryopreservation","alternate_names":["세포 동결보존","냉동보존","cryobanking"],"type":"concept","description":"Cryopreservation is cooling and storing cells, tissues or organs at very low or freezing temperatures so they can be used later.","overview":"Cells are usually stored in liquid nitrogen (about −196 °C) with cryoprotectants. Freezing and thawing reduce the number of surviving cells; published studies on adipose SVF report reduced cell counts after cryopreservation while stem cell potency of surviving cells may be retained. Storage conditions, documentation and consent are regulated in most countries.","official_url":null,"image_url":null,"country":null,"region":null,"wikidata_id":null,"wikipedia_url":null,"verified":false,"scores":{"authority":64,"trust":0,"citation":0},"topic":{"slug":"cell-banking","name":"Cell Banking & Cryopreservation"},"url":"https://worldwiki.io/entity/cryopreservation","facts":[{"id":25,"predicate":"definition","label":"Definition (NCI)","value":"Cooling and storing cells, tissues or organs at very low or freezing temperatures to save them for future use","unit":null,"confidence":100,"valid_from":null,"valid_until":null,"verified_at":"2026-09-14 13:42:07","source_ids":[9]},{"id":26,"predicate":"effect_on_svf","label":"Effect on SVF (study title finding)","value":"A 2019 study reported that cryopreservation reduces SVF cell counts but not the stem cell potency of surviving cells","unit":null,"confidence":100,"valid_from":null,"valid_until":null,"verified_at":"2026-09-14 13:42:07","source_ids":[25]},{"id":27,"predicate":"systematic_review","label":"Systematic review","value":"A 2026 systematic review examined effects of cryopreservation on SVF viability in human adipose tissue","unit":null,"confidence":100,"valid_from":null,"valid_until":null,"verified_at":"2026-09-14 13:42:07","source_ids":[26]}],"relations":[{"type":"related_to","confidence":100,"target":{"id":15,"slug":"cell-viability","name":"Cell Viability","type":"concept","description":"Cell viability is the proportion of living, functional cells in a sample; it is a key quality measure for cell preparations such as SVF before and after processing or freezing.","url":"https://worldwiki.io/entity/cell-viability"}}],"questions":[{"slug":"can-svf-be-cryopreserved","question":"Can SVF be frozen and stored (cryopreserved)?","intent":"informational","language":"en","short_answer":"Yes. SVF can be cryopreserved in liquid nitrogen with cryoprotectants. Freezing and thawing reduce the number of living cells; a 2019 study reported lower cell counts after cryopreservation but preserved stem cell potency in surviving cells, and a 2026 systematic review examined viability effects across studies. Viability after thaw should be measured before clinical use.","url":"https://worldwiki.io/q/can-svf-be-cryopreserved"},{"slug":"what-is-cell-cryopreservation","question":"What is cell cryopreservation?","intent":"informational","language":"en","short_answer":"Cell cryopreservation is the process of cooling and storing cells, tissues or organs at very low or freezing temperatures so they can be saved for future use. Cells are mixed with a cryoprotectant, cooled in a controlled way and kept in liquid nitrogen. Some cells do not survive freezing and thawing, so viability is tested afterwards.","url":"https://worldwiki.io/q/what-is-cell-cryopreservation"},{"slug":"how-long-can-stem-cells-be-stored","question":"How long can stem cells be stored?","intent":"informational","language":"en","short_answer":"Cells stored properly in liquid nitrogen can remain viable for many years, and cord blood banks report successful use after more than a decade. Storage time matters less than the quality of freezing, uninterrupted temperature control and documentation. For adipose SVF specifically, long-term data are limited, so ask the bank for its own thaw-viability results.","url":"https://worldwiki.io/q/how-long-can-stem-cells-be-stored"}],"sources":[{"id":9,"title":"NCI Dictionary of Cancer Terms: cryopreservation","url":"https://www.cancer.gov/publications/dictionaries/cancer-terms/def/cryopreservation","publisher":"National Cancer Institute (NCI)","type":"government","publication_date":null,"accessed_at":"2026-09-14 13:42:07"},{"id":25,"title":"Cryopreservation of Stromal Vascular Fraction Cells Reduces Their Counts but Not Their Stem Cell Potency","url":"https://pubmed.ncbi.nlm.nih.gov/31942351/","publisher":"Plastic and Reconstructive Surgery – Global Open (2019)","type":"academic","publication_date":"2019-01-01","accessed_at":"2026-09-14 13:42:07"},{"id":26,"title":"Effects of Cryopreservation on Stromal Vascular Fraction Viability in Human Adipose Tissue: Insights from a Systematic Review","url":"https://pubmed.ncbi.nlm.nih.gov/42631789/","publisher":"Tissue Engineering and Regenerative Medicine (2026)","type":"academic","publication_date":"2026-01-01","accessed_at":"2026-09-14 13:42:07"},{"id":15,"title":"About Stem Cells — patient resource","url":"https://www.aboutstemcells.org/","publisher":"International Society for Stem Cell Research","type":"organization","publication_date":null,"accessed_at":"2026-09-14 13:42:07"},{"id":14,"title":"ISSCR Guidelines for Stem Cell Research and Clinical Translation","url":"https://www.isscr.org/guidelines","publisher":"International Society for Stem Cell Research","type":"academic","publication_date":null,"accessed_at":"2026-09-14 13:42:07"}],"providers":[],"published_at":"2026-09-14 13:42:07","updated_at":"2026-09-14 22:42:07","verified_at":null}