journal证据等级 B独立来源
证据页Quantitative, traceable determination of cell viability using absorbance microscopy
PLoS One
发布 2022年1月19日访问于 2026年9月20日更新 2026年9月20日
原始来源
https://pmc.ncbi.nlm.nih.gov/articles/PMC8769294/
pmc.ncbi.nlm.nih.gov
核验
| 核验 | 独立于主体的来源 |
| 证据等级 | B · 同行评审·专业机构 |
| 独立来源 | 与对象无关的独立来源 |
| 来源 | journal |
| 链接状态 | 最近检查时可访问 |
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引用页面 (4)
FactCell Viability: Definition of a viable cell = A cell with an intact membrane or the capacity for metabolism, motility, proliferation or response to stimuliFactCell Viability: Dye-exclusion principle = Trypan blue enters cells with damaged membranes and stains them blue; unstained cells are counted as viableFactCell Viability: Trypan blue measurement variability = Image-based trypan blue scoring depends on microscope optics, cameras and settings; heat-killed cells took up about 50% more dye than formaldehyde-fixed cells in a NIST studyFactCell Viability: Related standard = ISO 20391 (Biotechnology — Cell counting), cited in NIST work on traceable viability measurement