journal证据等级 B独立来源
证据页Cryopreservation and its clinical applications
Integrative Medicine Research (Jang TH et al.), via PubMed Central
访问于 2026年9月20日更新 2026年9月20日
原始来源
https://pmc.ncbi.nlm.nih.gov/articles/PMC5395684/
pmc.ncbi.nlm.nih.gov
核验
| 核验 | 独立于主体的来源 |
| 证据等级 | B · 同行评审·专业机构 |
| 独立来源 | 与对象无关的独立来源 |
| 来源 | journal |
| 链接状态 | 最近检查时可访问 |
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FactCryopreservation: Typical long-term storage temperature = About −196 °C (liquid nitrogen) °CFactCryopreservation: Main methods = Slow freezing (about 1 °C/min, low cryoprotectant concentration) and vitrification (40–60% cryoprotectant, rapid cooling, no ice crystals)FactCryopreservation: Common cryoprotective agents = DMSO, glycerol, and polymers such as polyethylene glycol and hydroxyethyl starchFactCryopreservation: Main causes of freezing injury = Ice forming inside cells (fast cooling) and osmotic stress from concentrated solutes (slow cooling)FactCryopreservation: Clinical applications = Oocytes and embryos, sperm, stem cells (embryonic, mesenchymal, hematopoietic), hepatocytes, blood products and bone marrow