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Quantitative, traceable determination of cell viability using absorbance microscopy

PLoS One

Published Jan 19, 2022accessed Sep 18, 2026Updated Sep 19, 2026
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https://pmc.ncbi.nlm.nih.gov/articles/PMC8769294/

pmc.ncbi.nlm.nih.gov

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Cited by (4)

FactCell Viability: Definition of a viable cell = A cell with an intact membrane or the capacity for metabolism, motility, proliferation or response to stimuliFactCell Viability: Dye-exclusion principle = Trypan blue enters cells with damaged membranes and stains them blue; unstained cells are counted as viableFactCell Viability: Trypan blue measurement variability = Image-based trypan blue scoring depends on microscope optics, cameras and settings; heat-killed cells took up about 50% more dye than formaldehyde-fixed cells in a NIST studyFactCell Viability: Related standard = ISO 20391 (Biotechnology — Cell counting), cited in NIST work on traceable viability measurement