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Cell Viability Assays (Assay Guidance Manual)

National Center for Biotechnology Information (NCBI Bookshelf), Riss TL et al.

Published May 1, 2013accessed Sep 18, 2026Updated Sep 19, 2026
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https://www.ncbi.nlm.nih.gov/books/NBK144065/

ncbi.nlm.nih.gov

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Cited by (10)

FactCell Viability: Common plate-reader assay classes = Tetrazolium reduction (MTT, MTS, XTT, WST-1), resazurin reduction, protease substrate (GF-AFC), ATP luciferase and real-time luminescent assaysFactCell Viability: Typical research uses = Measuring cell proliferation, testing compounds for cytotoxic effects, and as an internal control for viable cell number in multiplexed assaysFactCell Viability: ATP assay characteristics = Described as the fastest and most sensitive viability assay and less prone to artifacts, but it is an endpoint assay that lyses cellsAnswerHow is cell viability percentage calculated?AnswerWhat is an acceptable cell viability for cell therapy products?AnswerTrypan blue vs MTT: which cell viability assay should I use?AnswerWhy does cell viability drop after thawing cryopreserved cells?AnswerDoes higher cell viability mean better fat graft survival?Answer줄기세포 연구소, 의료기관에서는 어떤 일을 하나요?ArticleCell Viability: Definition, Assays, Acceptance Criteria and Limits of Interpretation