journalEvidence level BIndependent
Evidence pageCryopreservation and its clinical applications
Integrative Medicine Research (Jang TH et al.), via PubMed Central
accessed Nov 11, 2026Updated Nov 11, 2026
Original source
https://pmc.ncbi.nlm.nih.gov/articles/PMC5395684/
pmc.ncbi.nlm.nih.gov
Verification
| Verification | Independent of the subject |
| Evidence level | B · Peer-reviewed or professional body |
| Independent | Independent of the subject |
| Source | journal |
| Link status | Reachable at last check |
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Cited by (5)
FactCryopreservation: Typical long-term storage temperature = About −196 °C (liquid nitrogen) °CFactCryopreservation: Main methods = Slow freezing (about 1 °C/min, low cryoprotectant concentration) and vitrification (40–60% cryoprotectant, rapid cooling, no ice crystals)FactCryopreservation: Common cryoprotective agents = DMSO, glycerol, and polymers such as polyethylene glycol and hydroxyethyl starchFactCryopreservation: Main causes of freezing injury = Ice forming inside cells (fast cooling) and osmotic stress from concentrated solutes (slow cooling)FactCryopreservation: Clinical applications = Oocytes and embryos, sperm, stem cells (embryonic, mesenchymal, hematopoietic), hepatocytes, blood products and bone marrow